• br Design of donor plasmids If no

  2018-10-20

  

  Design of donor plasmids If no footprintless gene targeting is required or it is intended to introduce larger transgenes, donor plasmids have to be constructed that contain the homology arms flanking the transgenes or selection cassettes coupled to promoters of choice. Similarly, donor plasmids c

• Recent reports have indicated the functional significance of

  2018-10-20

  

  Recent reports have indicated the functional significance of FOXM1 in pluripotent cells. FOXM1 is required for the maintenance of pluripotency in mouse embryonal carcinoma (EC) cells, via direct regulation of Oct4 transcription (Xie et al., 2010). More importantly, FOXM1 is important for maintaining

• Following from our prior research

  2018-10-20

  

  Following from our prior research to derive new hESC lines under increasingly defined culture conditions (Fletcher et al., 2006) we sought to establish a facility within which our experience and evolving practice and reagents could be implemented to a GMP and professionally accredited standard. Here

• br Materials and methods br

  2018-10-20

  

  Materials and methods Acknowledgments We would like to thank Dr. Keisuke Okita and Prof. Shinya Yamanaka for providing the tranylcypromine for reprogramming. We thank I Bente Smith Thorup for excellent technical assistance in the cell culture. We thank Jeannette Schoene and Dirk A. Pflumm for

• We speculate that in vivo serum GH of pituitary

  2018-10-20

  

  We speculate that in vivo, serum GH of pituitary and placental origin may be responsible for the expansion of mammary stem/progenitor gonadotropin releasing hormone agonist during developmental windows when the mammary gland grows isometrically (intrauterine life, childhood). In adulthood, when GH s

• Tracking the maturation status of stem cell derived cardiac

  2018-10-20

  

  Tracking the maturation status of stem cell-derived cardiac myocytes by ratiometric analysis of the cTnI:ssTnI protein profile provides a tool to permit direct comparisons of myocyte developmental status from different cell lines and with using different culture methods and differentiation protocol

• br Results br Discussion Adrenergic

  2018-10-20

  

  Results Discussion α-Adrenergic receptors play a critical role in the regulation of cellular growth pathways, including hypertrophy and proliferation. G protein-coupled α1ARs are the predominant α-adrenergic subtype in the myocardium of most species (Brückner et al., 1985), and catecholamine r

• br Experimental Procedures br Author Contributions br

  2018-10-20

  

  Experimental Procedures Author Contributions Acknowledgments Introduction We recently described an animal model in which primary genetic lesions in bone marrow (BM) stromal dihydrofolate reductase resulted in abnormal hematopoiesis in mice and the rare development of acute leukemia (Raa

• A role for PI K controlling ENPs

  2018-10-20

  

  A role for PI3K controlling ENPs fits well with current ideas of how FGF, BMP, β-catenin, and FAT4 regulate ENP self-renewal and differentiation (Figure 4C). The ENPs all receive the differentiation promoting β-catenin signal from the ureteric bud, but only the synthase that receive a stromal signa

• br Experimental Procedures br Author Contributions br Acknow

  2018-10-20

  

  Experimental Procedures Author Contributions Acknowledgments Introduction With the first reports on generating human induced pluripotent stem dna pk (hiPSCs) from human cells (Takahashi et al., 2007; Yu et al., 2007), the controversy regarding the ethics of research involving human embr

• Recently new genome modifying methods using sequence

  2018-10-20

  

  Recently, new genome-modifying methods using sequence-specific endonucleases, such as zinc-finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 9 (CRISPR/Cas9) systems, were developed o

• Recent studies indicate that the canonical

  2018-10-20

  

  Recent studies indicate that the canonical Wnt pathway affects BMSC osteogenic differentiation. Wnt signaling can promote osteoblastic precursor differentiation into more differentiated osteoblasts and can serve as a negative regulator of adipogenesis (Glass et al., 2005; Krishnan et al., 2006). Thi

• br Acknowledgments The study is supported by

  2018-10-20

  

  Acknowledgments The study is supported by funds to W-Q.G. from the Chinese Ministry of Science and Technology (2012CB966800, 2013CB945600, and 2012CB967903), the National Natural Science Foundation of China (81130038 and 81372189), the Science and Technology Commission of Shanghai Municipality (P

• Fmoc-Leu-OH br Experimental Procedures br Author

  2018-10-20

  

  Experimental Procedures Author Contributions Introduction Frontotemporal dementia (FTD) refers to a group of neurodegenerative diseases caused by focal but progressive neuronal loss, astrogliosis, and spongiosis in the frontal and temporal cortices associated with abnormal intracellular acc

• In this study we have developed a three step protocol

  2018-10-20

  

  In this study, we have developed a three-step protocol to differentiate hESCs to endothelial progenitor endothelin receptor antagonist (EPCs) using human recombinant laminins that are biologically relevant (LN521, LN511, and LN421) to mimic the in vivo endothelial substrata. First, vascular cell lin

14956 records 997/998 page Previous Next First page 上5页 996997998